Fig. 4

SAMHD1 Regulation of the PI3K/AKT Signaling Pathway in the HEC-1-B Cells and Its Impact on Proliferation, Migration, and Invasion of Endometrial Cancer Cells.Note (A) Western blot analysis of the expression of EMT-related molecules in HEC-1-B cells after treatment with 740 Y-P or SC-79; (B) CCK-8 assay to evaluate the proliferation rate in SAMHD1-silenced HEC-1-B cells upon treatment with 740 Y-P/SC-79. (C-D) Transwell assay to assess the migration and invasion capabilities (50 μm) of SAMHD1-silenced HEC-1-B cells treated with 740 Y-P/SC-79. Multiple comparisons were analyzed using one-way ANOVA. (These data are represented as quantitative data, using mean ± standard deviation. Group comparisons were conducted using one-way analysis of variance, while different time points were analyzed using two-way analysis of variance. Blue * indicates significance compared to the sh-NC + DMSO group (p < 0.05), whereas pink * indicates significance compared to the sh-SAMHD1 + DMSO group (p < 0.05). Cell experiments were repeated three times