Fig. 6

Impact of SAMHD1 on the PTEN phosphatase activity mediated by TRIM27-induced PTEN ubiquitination modification Note (A) Western blot detection of the ubiquitination levels of PTEN in the normal immortalized endometrial stromal cell line SHT290 and the endometrial cancer cell line HEC-1-B; (B) Western blot detection of the effect of SAMHD1 on PTEN ubiquitination; (C) Western blot detection of the effect of SAMHD1 on PTEN Ub-k48 and Ub-k63 ubiquitination; .(D) Expression of TRIM27 in TCGA-UCEC; (E) Western blot detection of the regulation of PTEN Ub-k27 ubiquitination by SAMHD1; (F) The correlation between the expression of SAMHD1 and TRIM27 in TCGA-UCEC is depicted by plotting a set of data points and drawing a red trend line to represent the relationship trend between the variables; (G) Western blot detection of the effect of SAMHD1 on TRIM27 expression; (H) Western blot detection of the effect of SAMHD1 on PTEN Ub-k27 ubiquitination mediated by TRIM27; (I) Western blot detection of the efficiency of siTRIM27; (J) CCK8 to detect the cell viability after silencing of TRIM27; (K) Western blot detection of the effect of silencing TRIM27 on SAMHD1-mediated PTEN Ub-k27 ubiquitination; (L) Immunopurification to obtain ubiquitinated PTEN and non-ubiquitinated PTEN; (M) ELISA to detect the effect of SAMHD1 on PTEN phosphatase activity (the data are presented as mean ± standard deviation, and the experiment was repeated three times). Data comparison between the two groups was analyzed using independent samples t-test, while multiple groups were analyzed using one-way analysis of variance. Different time points were analyzed using two-way analysis of variance, with * indicating comparison with the first group in each result, and p < 0.05 signifying statistical significance