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Fig. 2 | Biology Direct

Fig. 2

From: miR-155 mediated regulation of PKG1 and its implications on cell invasion, migration, and apoptosis in preeclampsia through NF-κB pathway

Fig. 2

miR-155 promotes apoptosis in human trophoblast-derived cell lines. Note: A Prediction of the binding sites of PKG1 and miR-155 from the miRDB website; B Confirmation of the interaction between PKG1 and miR-155 by dual-luciferase reporter gene assay; C Validation of the interaction between PKG1 and miR-155 through RNA pull-down analysis; D Detection of PKG1 mRNA expression in mouse placental tissues by RT-qPCR; E Evaluation of PKG1 protein expression in mouse placental tissues by Western Blot, n = 6; F Measurement of PKG1 mRNA expression in H/R-treated HTR-8/SVneo cells by RT-qPCR; G Analysis of PKG1 protein expression in H/R-treated HTR-8/SVneo cells by Western Blot; H Assessment of PKG1 mRNA expression in HTR-8/SVneo cells transfected with miR-155 inhibitor by RT-qPCR; I Determination of PKG1 protein expression in HTR-8/SVneo cells transfected with miR-155 inhibitor by Western Blot; J Quantification of PKG1 mRNA expression in H/R-treated BeWo cells by RT-qPCR; K Examination of PKG1 protein expression in H/R-treated BeWo cells by Western Blot; L Measurement of PKG1 mRNA expression in BeWo cells transfected with miR-155 inhibitor by RT-qPCR; M Analysis of PKG1 protein expression in BeWo cells transfected with miR-155 inhibitor by Western Blot. Cell experiments were repeated three times (n = 3). * indicates a statistical significance of p < 0.05 between the two groups

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Biology Direct

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