Fig. 4

Interaction of lncRNA CRNDE with YTHDC1 inhibits apoptosis in SH-SY5Y cells. (A) Pull-down experiment to detect the interaction between lncRNA CRNDE and YTHDC1. (B) RIP assay to measure the expression of lncRNA CRNDE. (C, D) Western blot analysis of YTHDC1 protein expression. (E) Flow cytometry analysis of apoptosis rate. (F) Western blot analysis of Bcl-2, Bax, and Caspase-3 proteins. (G) Levels of inflammatory factors TNF-α, IL-1β, IL-6, and IL-10. (H) qRT-PCR analysis of mRNA levels for autophagy-related markers LC3, Beclin1, and p62. (I) Western blot analysis of autophagy-related proteins LC3II/I, Beclin1, and p62. *P < 0.05 indicates a significant difference compared to the sh-NC group. #P < 0.05 indicates a significant difference compared to the sh-lncRNA CRNDE + oe-NC group. All data are expressed as Mean ± SD. Comparisons between two groups were performed using the unpaired Student’s t-test, while one-way ANOVA and Tukey’s post-hoc test were used for comparisons among multiple groups. All the experiments were repeated thrice