Fig. 5

Overexpression of YTHDC1 promotes ATG10 expression. (A) RIP assay revealed a reduction in ATG10 expression upon inhibition of lncRNA CRNDE. (B) qRT-PCR analysis of ATG10 mRNA levels. (C) Western blot analysis of ATG10 protein levels. (D) RNA stability assay in neuroblastoma cells treated with actinomycin. *P < 0.05 indicates a significant difference compared to the sh-NC group. #P < 0.05 indicates a significant difference compared to the sh-lncRNA CRNDE + oe-NC group. All data are expressed as Mean ± SD. Comparisons between two groups were performed using the unpaired Student’s t-test, while one-way ANOVA and Tukey’s post-hoc test were used for comparisons among multiple groups. All the experiments were repeated thrice