Fig. 4

Morphology of S. eriocheiris and expression of the cytoskeleton protein MreB after treatment with Phalloidin and A22. (A, B, C) S. eriocheiris's TEM negative staining images. A, using S. eriocheiris as a negative control. B, the length of S. eriocheiris was significantly increased after treatment with Phalloidin. C, the morphology of S. eriocheiris transformed from a spiral shape to an almost spherical shape following treatment with A22. (D, E, F) The gray value of MreB3, MreB4 and MreB5 in S. eriocheiris, following treatment with Phalloidin and A22, D and F did not exhibit significant alterations in expression levels. E, the expression of MreB5 was significantly reduced after A22 treatment of S. eriocheiris. (G) Western blot analysis of the expression of the skeleton protein MreB, SPE-0313 was used as reference. 1: S. eriocheiris without any treatment as control. 2: MreB expression in S. eriocheiris after treatment with Phalloidin. 3: MreB expression in S. eriocheiris after treatment with A22. (*: p < 0.05)