Fig. 5
The impact of Phalloidin and A22 on the S. eriocheiris growth. (A) The alterations of the medium color of S. eriocheiris after treatment with Phalloidin and A22. I: S. eriocheiris as negative control. II: S. eriocheiris was treated with 2.5 µg/mL Phalloidin for 12 h, the medium color changed from red to yellow. III: S. eriocheiris after treatment with 200 µg/mL A22 for 12 h, the medium color slightly changed. (B) Calculating the number alterations of S. eriocheiris in the first three groups presented in 5A using CCU method. I: In the negative control group, the number of S. eriocheiris can reach 108. II: The number of S. eriocheiris treated with Phalloidin could also reach 108, without alterations. III: The number of S. eriocheiris was reduced after 12 h treatment with A22, and only reached 106. (C) The front of three groups of S. eriocheiris were cultivated for 8 days, and calculated the alterations of number by CCU method. I: Negative control group. II: The number of S. eriocheiris could also reach 108 after treatment with Phalloidin, without alterations. III: The number of S. eriocheiris treated with A22 could also reach 108 after culture for 8 days