Fig. 7

Blood lymphocytes morphology of E. sinensis under phase contrast microscope. (A) The column results show that the blood lymphocytes of E. sinensis were infected with wild type S. eriocheiris and S. eriocheiris treated with Phalloidin and A22 after 0 h, the hemolymph cells were basically normal fusiform or ellipsoid shape. (B) Compared with the negative control group, the other three groups of S. eriocheiris showed a small number of cellular debris after the blood lymphocytes were infected for 12 h, but the morphology of the blood lymphocytes was basically maintained in a normal state. (C) After 24 h of infection, the number of blood lymphocytes in the Phalloidin group was significantly reduced. Compared with the positive control group, the survival rate of blood lymphocytes in the A22-treated group was increased, and the surviving cells maintained the morphology of the normal growth state