Fig. 2

miR-630 inhibits the self-renewal and maintenance of PCSCs. A, Expression of miR-630 in PCSCs after the treatment of miR-630 mimic/inhibitor were determined by RT-qPCR; B, Detection of cell proliferation by EdU and the positive expression rate in each group; C, The cell colony formation and the number of cell clones in each group; D, The sphere formation in each group; E, The expression of Nanog and Oct4 of cells in each group were determined by RT-qPCR; F, The protein expression of Nanog and Oct4 of cells in each group were determined by western blot analysis; * p < 0.05 vs. the mimic-NC group, # p < 0.05 vs. the inhibitor-NC group; miR-630, microRNA-630; PCSCs, pancreatic cancer stem cells; EDU, 5-ethynyl-2’-deoxyuridine; RT-qPCR, Reverse transcription quantitative polymerase chain reaction; NC, negative control. Measurement data was expressed by mean ± standard deviation. Comparison between two groups was analyzed by independent sample t-test. The experiment was repeated for three times