Fig. 3

BRD4 and p63 regulate the expression of selected target genes. a–c Schematic representation of the regulatory regions of the human HK2, FOXM1 and EVPL genes in which putative p63 binding sites are highlighted. ChIP-Seq analysis using GSE140992 and GSE33571 datasets revealed the presence of BRD4 and p63 bound to human HK2, FOXM1 and EVPL genes. TSS: transcription start site. The scheme was drawn using JASPAR (http://jaspar.genereg.net/). d–f ChIP analyses were carried out with specific antibodies for BRD4 and p63. Data are shown as mean ± SD of two independent experiments (unpaired Student’s t test). g–i HEKn cells were silenced with siSCR, sip63 and two different siRNA for BRD4 for 48 h. Then RT-qPCR analyses were carried out to evaluate the mRNA levels of HK2, FOXM1 and EVPL. Graphs present means ± SD of three independent experiments (unpaired Student’s t test). j–l) HEKn cells were silenced with siSCR, sip63 and two different siRNA for BRD4 for 48 h. Then Western Blot analysis were carried out to evaluate the protein levels of HK2, FOXM1 and EVPL. The blot is representative of three independent experiments