Fig. 2

R1881 of LNCaP cells treatment increases protein levels involved in translation. A Differentially expressed genes from 1 nM R1881, 1 nM R1881 + 10 µM enzalutamide treatment compared to DMSO were ranked based on their expression value and significance. GSEA was performed using the pre-ranked protein lists, and a heatmap was generated based on the normalised enrichment score and significance of GSEA. Translational pathways were enlarged to show details. B Representative western blot of the influence of DMSO (CTRL), 1 nM R1881 treated LNCaP cells combined with DMSO (vehicle control) or 20 µg/ml Cycloheximide (C6), and 20 µg/ml Cycloheximide (C6) alone in LNCaP cells after 0 h, 2 h, 4 h, and 6 h. Uncropped western blots are displayed in Supplementary Fig. 3. C Densitometry of AR protein levels relative to TPS. Relative expression levels compared to CTRL after treatment were shown as mean ± SD of five independent experiments. All differences highlighted by asterisks were statistically significant (*: p ≤ 0.05; **: p ≤ 0.01; ***: p ≤ 0.001)