Fig. 5
From: The impact of androgen-induced translation in modulating androgen receptor activity
Influence of different concentrations of Rocaglamide on AR protein levels and transactivity in LNCaP cells. A Representative western blot to investigate the influence of different concentrations of Briciclib (eIF4E-inhibitor), Rocaglamide (Roca, eiF4A-inhibitor), and SBI-0640756 (eIF4G1-inhibitor) on the influence of R1881-induced increase of AR protein. In addition, p-eIF4E and eiF4E were investigated to assess the changes in eiF4F activity. Uncropped western blots are displayed in Supplementary Fig. 7. B Densitometry of AR protein levels relative to TPS. Relative expression levels compared to CTRL after treatment were shown as mean ± SD of five independent experiments. All differences highlighted by asterisks were statistically significant (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). C Densitometry of eiF4E protein levels relative to TPS. Relative expression levels compared to CTRL after treatment were shown as mean ± SD of five independent experiments. Relative expression levels after treatment were shown as mean ± SD of five independent experiments. D Densitometry of p-eiF4E protein levels relative to eiF4E. Relative expression levels compared to CTRL after treatment were shown as mean ± SD of five independent experiments. All differences highlighted by asterisks were statistically significant (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). E Representative western blot to investigate the influence of different concentrations of Rocaglamide on the influence of R1881-induced increase of AR protein. F Densitometry dose–response curves of AR protein levels relative to TPS. Relative expression levels compared to CTRL after treatment were shown as mean ± SD of five independent experiments. G Relative changes of AR transactivity to determine the influence of different concentrations of Rocaglamide on the influence of R1881-induced increase of AR transactivity. Changes were assessed by changes in KLK3 normalised to HPRT1 mRNA determined by qPCR. Relative expression levels after treatment were shown as mean ± SEM of five independent experiments