Fig. 2

FXR1 knockdown inhibited the proliferation of ESCA cells. (A) ESCA cells were transduced with lentivirus that express two different shRNAs targets FXR1 (KD1 and KD2), control shRNA (NC), or orf (OE) along with empty vector (EV), and western blot was performed 72 h after transduction using antibodies indicated. β-actin is used as a loading control. (B) ESCA cells were transduced using the FXR1 shRNAs, and cell viability was determined using the CCK-8 assay at indicated time points. Each experiment was performed for 5 days and in triplicates. (C) ESCA cells were transduced using the FXR1 shRNAs and subjected to a 6–8 days’ colony formation assay. The number of colonies was calculated. (D) ESCA cells were transduced using the FXR1 orf (OE), and cell viability was determined using the CCK-8 assay at indicated time points. Each experiment was performed for 5 days and in triplicates. (E) ESCA cells were transduced using the FXR1 orf (OE) and subjected to a 6–8 days colony formation assay. The number of colonies was calculated. Scale bar, 100 mm. Data are presented as the mean ± SEM (n = 3 in each group). *P < 0.05, **P < 0.01