Fig. 7

ATOH8 overexpression inhibited the proliferation and migration of ESCA cell lines. (A) ESCA cells were transfected using the ATOH8 overexpression, and cell viability was determined using the CCK-8 assay at indicated time points. Each experiment was performed for 5 days and in triplicates. (B) ESCA cells were transfected using the ATOH8 overexpression and subjected to a 6–8 days colony formation assay. The number of colonies was calculated. Scale bar, 100 mm. (C) ESCA cells were transfected with ATOH8 overexpression followed by Transwell assay for cell migration. The cells were then observed microscopically (magnification, x200). The relative images and accompanying statistical plots were presented. The experiments were performed in triplicate. (D) The expression levels of EMT-related proteins were detected in ATOH8 overexpressed cells by Western blot. β-actin is used as a loading control. (E) qRT-PCR analysis revealed the half-life of ATOH8 mRNA in control or FXR1 knockdown cells after treatment with Actinomycin D. Data is presented as the mean ± SEM (n = 3 in each group). *P < 0.05, **P < 0.01