Fig. 4

Downstream target genes analysis of m6A regulators in HCC. A, Visualization of the downstream target genes correlated with harmful m6A regulators across 31 datasets. The gene node size represents the cumulative correlation between the corresponding target gene and harmful m6A regulator. The red line indicates negative correlation and the blue line signifies positive correlation. B, Main KEGG enrichment-pathways of the target genes associated with harmful m6A regulators. C, Main GO enrichment-pathways of the target genes associated with harmful m6A regulators. D, Relationship between CDK4 and 3 harmful m6A regulators among 29 datasets. E–G, Correlation of CDK4 with HNRNPC, YTHDF1 and IGF2BP3 in LICA-FR. H, Single-cell sequencing analysis of CDK4 expression in various cells in GSE202642. I, CDK4 protein expression in the human protein atlas. J, Relationship between CDK4 and HCC-related signatures among 29 datasets. K–M, Association of CDK4 with vascular invasion inhibition, proliferation and metastasis signatures in GSE112790. Cor, correlation coefficient. Negative, negative correlation. Positive, positive correlation. TEC, tumor-associated endothelial cells. CAF, cancer-associated fibroblasts. TAM, tumor-associated macrophages. HPC-like, cells with an unknown entity but express hepatic progenitor cell markers. *P < 0.2 and |Cor|>0.1, **P < 1e-6 and |Cor|>0.1, ***P < 1e-11 and |Cor|>0.1