Fig. 5
From: TRIM47 drives gastric cancer cell proliferation and invasion by regulating CYLD protein stability
TRIM47 promotes proliferation, invasion, and migration of gastric cancer cells by activating the NF-κB pathway. (A) Gene Set Enrichment Analysis (GSEA) results. (B) Western blot analysis of key molecules in the NF-κB pathway, such as p-P65, P65, p-IκBα, and IκBα. (C) CCK8 assay to assess proliferation of AGS and HGC-27 cells stably overexpressing TRIM47 after treatment with BAY 11-7085 (10µmol/L) for 24 h. (D, E) Edu assay to evaluate proliferation of the mentioned cells. (F, G) Wound healing assay to measure cell migration. (H, I) Transwell assay to determine cell migration and invasion capabilities. (J) Western blot analysis of p-P65, P65, N-cadherin, E-cadherin, Occludin, and Snail1 protein expression in cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001, t-test