Fig. 6
From: TRIM47 drives gastric cancer cell proliferation and invasion by regulating CYLD protein stability
TRIM47 interacts with CYLD. (A) Exploration of proteins possibly interacting with TRIM47 using the BioGRID database. (B) Co-immunoprecipitation (Co-IP) assay confirming the interaction between TRIM47 and CYLD in HEK HEK-293T cells. (C) Co-IP assay confirming the interaction between TRIM47 and CYLD in AGS and HGC-27 cells. (D) mRNA expression of CYLD in TRIM47 knockdown and overexpressing gastric cancer cells. (E) Protein expression of CYLD in TRIM47 knockdown and overexpressing gastric cancer cells. (F) Expression of CYLD protein in gastric cancer cells transfected with different concentrations of TRIM47 overexpression plasmid. (G, H) Expression of CYLD protein in gastric cancer cells transfected with specified plasmids and treated with cycloheximide (CHX) for specified durations