Fig. 7
From: TRIM47 drives gastric cancer cell proliferation and invasion by regulating CYLD protein stability
TRIM47 Induces Lys48-Linked Polyubiquitination of CYLD. (A) Expression of CYLD in DMSO, CQ, or MG-132-treated stable TRIM47-transfected gastric cancer cells. (B) Verification of CYLD protein ubiquitination levels after LV-NC or LV-TRIM47 transfection in gastric cancer cells. (C) Ubiquitylation of CYLD detected in HEK-293T cells transfected with Flag-TRIM47, Myc-CYLD, and Ub-k48R or Ub-63R mutant plasmids. (D) Ubiquitylation of CYLD detected in HEK-293T cells transfected with Flag-TRIM47, Myc-CYLD, and Ub-k48 plasmids. (E) Ubiquitylation of CYLD detected in HEK-293T cells transfected with TRIM47-SH #1 or 2, Myc-CYLD, and Ub-k48 plasmids