Fig. 4
RNF19A negatively regulated the AKT/mTOR signalling pathway in BCa. (A) Volcano plot showing the differentially expressed genes between the RNF19Ahigh and RNF19Alow groups. (B) KEGG enrichment analysis of RNF19A-related DEGs. (C) GSEA plots depicting the enrichment of ubiquitin-mediated proteolysis in samples with high RNF19A expression. (D) GSEA plots depicting the enrichment of the mTOR signalling pathway in samples with low RNF19A expression. (E-L) Correlation analyses of RNF19A expression and the expression levels of multiple genes in the mTOR signalling pathway, including EIF4EBP1, PGF, RPS6KB2, MLST8, PIK3CD, VEGFB, PIK3R5, and EIF4E2. (M-O) Expression of proteins involved in mTOR signalling in T24 and 5637 cells stably overexpressing RNF19A. (P-R) Protein levels of AKT, p-AKT T473, mTOR, p-mTOR S2448, S6K1, and p-S6K1 T389 in T24 and 5637 cells after RNF19A was knocked down. *P < 0.05, **P < 0.01, and ***P < 0.001