Fig. 6

RNF19A regulated the activation of AKT/mTOR signalling via ILK. (A-C) western blot analysis of the levels of phosphorylated AKT, mTOR, and S6K1 in T24 and 5637 cells transfected with an siRNA targeting ILK and quantitative analyses. (D-F) western blot analysis of phosphorylated AKT, mTOR, and S6K1 levels in BCa cells overexpressing ILK and quantitative analyses. (G-I) Protein levels of AKT, p-AKT, mTOR, p-mTOR, S6K1, and p-S6K1 in RNF19A-overexpressing BCa cells after transfection with the ILK overexpression plasmid or empty vector and quantitative analyses. (J-L) western blot results showing that the increased levels of phosphorylated AKT, mTOR, and S6K1 induced by RNF19A knockdown were eliminated after silencing ILK. *P < 0.05, **P < 0.01, and ***P < 0.001