Fig. 5

YTHDF2 promoted tumor growth and metastasis in vivo. A, B The tumor growth curve of xenografts was plotted by measuring the tumor size (width^2 × length × 0.52) with caliper. The tumor size at the endpoint in the above group was analyzed by Student’s t-test. The subcutaneous tumor models were observed after 28 days, the anatomized subcutaneous tumor xenografts were weighed and analyzed with Student’s t-test. C Representative IHC staining micrographs of YTHDF2 in tumor xenografts were conducted. D. Proteins were extracted from animal subcutaneous tumors for WB detection of EMT-associated proteins. E. The BALB/c nude mice injected with cells via tail vein were imaged at week 4 to evaluate the entire metastasis by the IVIS. F. H&E staining of multiple metastatic organs was performed to identify the metastatic loci. Error bars represent the SD obtained from at least three independent experiments.*P < 0.05, **P < 0.01, ***P < 0.001