Fig. 2

Deletion of miR-31 protects against experimental sepsis in mice. A. The deletion of miR-31 in mice increased the survival rate of mice with CLP (21-gauge needle)-induced sepsis (the data are presented as the mean ± SEM, n = 8, mice per group; **P < 0.01, Kaplan–Meier survival analysis). B. Effects of miR-31 deletion on murine sepsis score (MSS) in mice with sepsis. Sepsis was induced by CLP (21-gauge needle) (the data are presented as the mean ± SEM, n = 8, mice per group; **P < 0.01). C and D. Lung tissues of mice with CLP-induced sepsis were stained with hematoxylin and eosin (12 h; scale bars, 50 μm), and the histopathologic lung injury score in miR-31^−/− and WT mice were assessed. E. The mRNA expression of Arg1 in lung tissues of WT and miR-31^−/− mice with CLP-induced sepsis were assayed. F. Immunofluorescence staining of lung sections for the macrophage marker F4/80 (green), Arg1(red) and DAPI (blue) (12 h; scale bar, 50 μm). G. The mRNA expression of IL-10, IL-1β, IL-6 and TNF-α in PBMCs of mice with CLP were detected.The data are presented as the mean ± SEM, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001