Fig. 4

Chi3l1, downstream of miR-31, promotes M2 macrophage polarization. A. The result of RNA-seq in PEMs isolated from WT and miR-31^−/− mice stimulated with 100 ng/ml LPS for 48 h. B. The mRNA expression of Chi3l1 in Chi3l1-overexpressing PEMs was assayed. C. The mRNA expression of Chi3l1 in Chi3l1-underexpressing PEMs was measured. D. The Chi3l1 overexpression plasmid to increase the expression of Chi3l1 in PEMs isolated from WT mice, then the mRNA expression of iNOS, CD86, Arg1 and CD206 in PEMs with or without 100 ng/ml LPS for 48 h were detected. E. The siRNA was used to inhibit the expression of Chi3l1, the mRNA expression of iNOS, CD86, Arg1 and CD206 in Chi3l1-underexpresssing PEMs stimulated with or without 100 ng/ml LPS for 48 h were assayed. The data are presented as the mean ± SEM. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001