Fig. 5

miR-31 regulates macrophage polarization to M2 through Chi3l1. A. The mRNA expression of Chi3l1 in PBMCs of miR-31^−/− mice with CLP was assayed. B, C and D. The mRNA and protein expression of Chi3l1 in PEMs isolated from miR-31^−/− mice stimulated with 100 ng/ml LPS for 48 h were detected. E. The Chi3l1 overexpression plasmid to increase the expression of Chi3l1 in PEMs isolated from WT and miR-31^−/− mice, then the mRNA expression of Arg1 and iNOS in PEMs with or without 100 ng/ml LPS for 48 h were detected. F, G and H. The mRNA and protein expression of Chi3l1 in miR-31-5p-overexpressing PEMs stimulated with 100 ng/ml LPS for 48 h were assayed. I. miR-31-5p mimic was used to increase the expression of miR-31-5p, then the mRNA expression of Arg1 and iNOS in Chi3l1-overexpresssing PEMs stimulated with or without 100 ng/ml LPS for 48 h were assayed. The data are presented as the mean ± SEM. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001