Fig. 6

miR-31-5p directly inhibits CHI3L1. A. Complementary sequences between hsa-miR-31-5p and the 3′ UTR of CHI3L1. B. CHI3L1 promoter luciferase reporter (CHI3L1-Luc) was co-transfected with miR-31-5p mimic in 293T cells for 24 h, and then relative luciferase activity was analyzed. C. The siRNA was used to inhibit the expression of Ago2, CHI3L1 promoter luciferase reporter (CHI3L1-Luc) was co-transfected with miR-31-5p mimic in Ago2-underexpressing 293T cells for 24 h, and then relative luciferase activity was analyzed. D, E, F and G. The association between Chi3l1, mmu-miR-31-5p and Ago2 was ascertained by analyzing PEMs lysates using RNA immunoprecipitation with an Ago2 antibody. qPCR was used to detect the change of Chi3l1 (D, E) and mmu-miR-31-5p (F, G) levels. The data are presented as the mean ± SEM. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001