Fig. 9

Relationship of CCNA2 to cell proliferation, migration, cell cycle, and apoptosis. (A) Protein levels of CCNA2 in HCC and paraneoplastic tissues. (B) Immunohistochemistry detected the protein expression level of CCNA2 in normal, paraneoplastic, and tumor tissues. (C) Protein expression levels of CCNA2 in normal and two HCC mouse models. (D) Protein expression and transcriptomic levels of CCNA2 in normal hepatocytes and tumor cell lines as demonstrated by the western blot and qRT-PCR. (E) Protein expression and mRNA levels of silenced CCNA2 are shown by the western blot and qRT-PCR. (F) Representative images on Edu staining and quantitative analysis. (G) Representative correlation image of the scratch experiment (H-I) quantitative analysis of the Edu staining, the scratch assay on 24 and 48 h. (J)Temporal fold plot of cell proliferative capacity at different periods as detected by CCK8 assay. (K) Plot of cell flow cycle changes and quantitative histograms for each cell cycle. (L) CDK1 protein expression levels after silencing of CCNA2 as detected by the western blot assay. (M) Relative protein degrees of apoptosis-related markers (Bax, Bcl2, and P53). (N-O) Relative protein levels of EMT-related markers (MMP7, MMP9, N-cadherin, E-cadherin, ZO-1, β-catenin, and vimentin). (P) Relative protein levels of PI3K/AKT pathways. *p < 0.05, **p < 0.01,*** p < 0.001, ns No Significance