Fig. 1
From: Soft X-ray tomography analysis of mitochondria dynamics in Saccharomyces cerevisiae
Workflow of soft X-ray tomography for yeast cells. Yeast cells were stained with mitochondria dye DiOC6(3) and mixed with 100Â nm nano-gold particles. Glow discharged grids were premarked with nano-gold particles. Yeast and nano-gold samples were seeded on both sides of the grids. Next, grids were blotted with filter paper and frozen in liquid ethane using a plunge freezer. After freezing, cryo-samples were screened using a cryo-stage fluorescence microscope to select the regions of interest for further SXT analysis. IMOD and Fiji (ImageJ) software were used to reconstruct SXT images. IMOD software was used to build 3D segmented models