Fig. 2

Knockdown of circSCP2 suppressed proliferation, invasion and migration of CRC cells. (A) qRT-PCR analysis of circSCP2 mRNA expression among normal colonic epithelial cells (NCM460) and CRC cells (SW480, SW620, Colo320, HCE8093, DLD1, HCT116, RKO, LOVO). (B) qRT-PCR analysis was used to evaulate the transfection efficiency of three types of siRNAs in colorectal cancer cells (RKO and LOVO cells). (C) CCK8 assays and (D) EdU analysis were used to determine the growth ability of CRC cells after knocking down the expression of circSCP2 relative to the negative control group. (E) Wound healing assays were used to evaluate the effect of circSCP2 knockdown on the invasion ability of RKO and LOVO cells. (F) Transwell experiments were used to evaluate the effect of circSCP2 knockdown on the migration and invasion ability of RKO and LOVO cells. (G) Colony formation assays were conducted to evaluate the proliferation ability of CRC cells after knocking down of circSCP2. The data were independently repeated at least three times. The results were presented as the means ± SD. Data were compared using one-way ANOVA with Dunnett’s multiple comparisons test