Skip to main content
Fig. 6 | Biology Direct

Fig. 6

From: Exosomes containing circSCP2 in colorectal cancer promote metastasis via sponging miR-92a-1-5p and interacting with PTBP1 to stabilize IGF2BP1

Fig. 6

circSCP2 interacted with PTBP1 and regulateed its ubiquitination-induced degradation in CRC cells. (A) Venn diagram showed the intersection of RBPs predicted by the 4 online databases (circAtlas, RBPDB, starbase and catRAPID). (B) Silver-stained SDS-PAGE gel-containing proteins derived from RNA pulldown by circSCP2 probe and negative control. (C) Western blotting analysis of protein precipitates obtained from RNA pulldown by circSCP2 probe and negative control. (D) qRT-PCR analysis of circSCP2 expression in anti-PTBP1 antibody-incubated precipitation relative to anti-IgG negative control. (E) qRT-PCR analysis of PTBP1 mRNA expression in circSCP2 OE SW480 and RKO cells. (F) Western blotting analysis of PTBP1 protein and EMT-related proteins including E-cadherin, N-cadherin and Vimentin in circSCP2 OE (SW480 and RKO) cells as well as cirSCP2-knockdown (RKO and LOVO) cells. (G) Western blotting analysis of PTBP1 protein in control and circSCP2-knockdown RKO cells as well as circSCP2 OE SW480 cells treated with CHX treatment. (H) Ubiquitination modification of PTBP1 proteins in circSCP2 OE SW480 cells and circSCP2-knockdown RKO cells was determined via Co-IP and western blotting. Data were compared using Student’s t- test

Back to article page

Biology Direct

ISSN: 1745-6150

Contact us