Fig. 1
Inhibition of MLL1 expression ameliorates symptoms of PE mice and alleviates ferroptosis in placental tissues. A murine model of PE was established by injection of sFlt-1 adenovirus via tail vein at E8.5, and the control mice were injected with adenovirus encoding Fc protein, followed by injection of sh-MLL1 adenovirus, with sh-NC adenovirus as the control. A: Measurement of blood pressure in mice, n = 12. B: Detection of proteinuria in urine, n = 12. C: Weight of placenta and fetus, n = 12. D: Detection of MLL1 expression in placenta by qRT-PCR, n = 6. E: Observation on the pathological structure of placenta and kidney by H&E staining and PAS staining, respectively. The black arrow indicates the placental villous vascular interstitium; ▲ indicates trophoblasts; the green arrows indicates pathological features of glomeruli in renal tissues, n = 6. F-I: Detection of ROS, MDA, Fe2+, and GSH levels in placenta, n = 6. J: Detection of MLL1, ASCL4, GPX4, and FTH1 expressions in placenta by Western blot, n = 6. Data in panels BCDFGHI were analyzed by one-way ANOVA, and data in panels AJ were analyzed by two-way ANOVA, followed by Tukey’s multiple comparisons test. **P < 0.01