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Fig. 3 | Biology Direct

Fig. 3

From: MLL1 promotes placental trophoblast ferroptosis and aggravates preeclampsia symptoms through epigenetic regulation of RBM15/TRIM72/ADAM9 axis

Fig. 3

MLL1 promotes RBM15 expression by increasing H3K4me3 modification; RBM15 enhances m6A modification to promote YTHDF2 binding to TRIM72 mRNA and degrade TRIM72, thereby inhibiting TRIM72 expression. A: Analysis of the enrichment of MLL1 and H3K4me3 on RBM15 promoter in tissues (n = 6) and cells (n = 3) by ChIP. B-C: Detection of RBM15 and TRIM72 expressions in tissues (n = 6) and cells (n = 3) by qRT-PCR and Western blot. D: Quantitative analysis of m6A modification in tissues (n = 6) and cells (n = 3). E: Analysis of the m6A modification on TRIM72 mRNA and the enrichment of YTHDF2 in tissues (n = 6) and cells (n = 3) by RIP. F: Detection of TRIM72 mRNA stability in cells (n = 3) by actinomycin D; HTR-8/SVneo cells were transfected with sh-YTHDF2, with sh-NC as the control. G-H: Detection of YTHDF2 expression in cells (n = 3) by qRT-PCR and Western blot. Data in panels AEF were analyzed by two-way ANOVA, and data in panels BCD were analyzed by one-way ANOVA, followed by Tukey’s multiple comparisons test. Data in panels GH were analyzed by t test. **P < 0.01

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