Fig. 5
Inhibition of TRIM72 partially reverses the inhibitory effect of MLL1 inhibition on ferroptosis of HTR-8/SVneo cells. HTR-8/SVneo cells were transfected with sh-TRIM72, with sh-NC as the control. A: Detection of transfection efficiency by qRT-PCR; then HTR-8/SVneo cells were treated with sh-MLL1 for a combined experiment. B: Detection of TRIM72, ASCL4, GPX4, and FTH1 expressions in cells by Western blot. C: Detection of cell viability by CCK-8 assay. D-F: Detection of MDA, Fe2+, and GSH levels in cells by kits. G: Detection of ROS levels in cells by fluorescence. H: Detection of cell invasion and migration by Transwell. The cell experiments were repeated three times independently. Data in panel A were analyzed by t test. Data in panels CDEFGH were analyzed by one-way ANOVA, and data in panel B were analyzed by two-way ANOVA, followed by Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01