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Fig. 7 | Biology Direct

Fig. 7

From: USP44 regulates HEXIM1 stability to inhibit tumorigenesis and metastasis of oral squamous cell carcinoma

Fig. 7

USP44 enhances the stability of HEXIM protein. (A) Western blotting showing the expression of HEXIM in CAL-27 and SCC-9 cells. (B) Co-IP with anti-USP44 or anti-HEXIM1 antibody in CAL-27 and SCC-9 cells showing the endogenous interaction between USP44 and HEXIM1. (C) Co-IP with anti-Flag or anti-Myc antibody showing the interaction between Flag-USP44 and Myc-HEXIM1 overexpressed in 293T cells. (D) Effect of overexpression of USP44 on HEXIM1 stability. CAL-27 cells were transfected with either empty vector or USP44-expressing vector and then treated with CHX for the indicated periods of time. (E) Effects of USP44 overexpression on HEXIM1 ubiquitination. 293T cells transfected with Flag-USP44, Myc-HEXIM1 or HA-ubiquitin (Ub) plasmids were subjected to IP with anti-Myc antibody and then immunoblotted with the anti-HA or anti-Myc antibody

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Biology Direct

ISSN: 1745-6150

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