Fig. 2

Knockdown of RRP9 suppressed the proliferation and migration of BC cells. A. Screening for effective interference targets of RRP9 by qRT‒PCR. B-C, RRP9 knockdown in MDA-MB-231 and BT549 cells was confirmed by qRT‒PCR analysis (B) and Western blot (C) 48 h post-transfection. D-E, Cell proliferation was measured in MDA-MB-231 and BT549 cells at the indicated time points by a Celigo cell counting assay (D) and colony formation assay (E). F, The cell cycle distribution of MDA-MB-231 and BT549 cells upon RRP9 depletion was assessed via flow cytometry. G, Changes in the apoptosis rate of BC cells were induced by RRP9 depletion, as determined via flow cytometry. H, The impaired migration capacity of MDA-MB-231 and BT549 cells was evaluated via Transwell assays. **P < 0.01 and ***P < 0.001