Fig. 5
From: The RRP9-JUN axis promotes breast cancer progression via the AKT signalling pathway
RRP9 promoted proliferation and migration in BC cells by targeting JUN. A, The mRNA levels of RRRP9 and JUN were evaluated by qPCR to confirm successful transfection. B, The effects of RRP9 overexpression alone, JUN knockdown alone, or simultaneous RRP9 overexpression and JUN knockdown on the proliferation rate of MDA-MB-231 cells were assessed via a Celigo cell counting assay. C, Representative images (upper panel) and quantification of colony number (lower panel) determined via a colony formation assay. D, Apoptosis was assessed by flow cytometry. E, The migration capacity of MDA-MB-231 cells was examined by transwell assays. *P < 0.05, **P < 0.01 and ***P < 0.001