Fig. 6

RRP9 regulated cell proliferation and apoptosis in BC via the AKT signalling pathway A, Upstream regulatory pathways identified via IPA. Nodes are displayed using various shapes that represent the functional class of the gene. Arrows indicate the direction of signalling. The genes from the gene expression lists (focus molecules) are represented as red and green based on their fold change in expression. The downregulated genes are shown in green, and the upregulated genes are shown in red. B, Western blot was performed to assess the levels of proteins in the AKT signalling pathway. C, The percentages of apoptotic BT549 cells after RRP9 depletion or SC79 treatment were determined via flow cytometry. D, The protein and phospho-protein levels of AKT in BT549 cells after RRP9 depletion or SC79 treatment were detected via WB assays. *P < 0.05, **P < 0.01 and ***P < 0.001