Fig. 2

Cell types and specific marker genes identified in scRNA-seq datasets. (A) Major testis cell types corresponding to cell clusters in cattle testes in UMAP. (B) Heatmap showing of the relative expression of representative cell-specific marker genes in the 13 major cell types for single-cell RNA-seq datasets. Color bar indicates the relative expression level for selected genes. (C) Expression patterns of selected markers used to assign cell types projected on the UMAP plots. One cell marker for each cell type is shown in the Figure Red (or gray) indicates a high (or low) expression level as shown on the color key. (D) Distribution profiles of per-cell attributes compared across the 13 cell types. nGene, the number of detected genes per cell; nUMI, the number of Unique Molecular Identifiers (UMI) per cell; nCell, the number of cells within each cell type