Fig. 4

The developmental trajectory of cattle testicular germ cells. (A) Pseudotime trajectory order of cattle testicular germ cells. Each dot represents an individual cell. The color bar from dark to light indicates the start and end of the trajectory. (B) The successive development trajectory of testicular germ cells. Cells are colored to their predicted locations along pseudotime trajectory. Three discrete cellular states (pre-branch, cell fate 1, cell fate 2) during germ cell development. (C) Expression profile of the selected genes involved in cellular state along the pseudotime trajectory. Black line indicates expression tendency across germ cells. (D) Heatmap and GO terms enrichment analysis of the significantly differentially-expressed genes. The significantly DEGs were enriched on the three gene clusters showed by the heatmap analysis. Associated GO terms with each gene cluster is shown on the corresponding right