Fig. 4

Identification of peptides targeting PTGES3 protein. (A) Name and sequence of peptides bound to PTGES3 protein. (B) Name and sequence of synthetic peptides conjugated with FITC and TAT sequence (GRKKRRQRRRPPQQ). (C) CCK-8 showed the growth rate of Huh7 cells treated with six synthetic peptides (n = 3). (D) IF experiments showed the cellular localization of PTGES3 protein (red) and FITC labeled FIP-2 (green) in Huh7 cells (n = 3). (E) The optimized binding modes between PTGES3 protein and F-2, Blue: PTGES3 protein; Green: F-2 peptide. (F) Temperature-dependent cellular thermal shift assay displayed the elevated thermal stability of PTGES3 in HCC cell lysates treated with FIP-2 (n = 3). (G) Concentration-dependent cellular thermal shift experiment revealed the increased thermal stability of PTGES3 treated with FIP-2 in Hep3B and Huh7 cell lysates at 56 and 59°C, respectively (n = 3)