Fig. 6
From: PTGES3 proteolysis using the liposomal peptide-PROTAC approach
PTGES3-PROTAC induced PTGES3 proteolysis in a transcriptional independent manner. (A) Western blotting examination showed the PTGES3 degradation in HCC cells treated with different doses of FIP-2 or PTGES3 degrader liposomes (n = 3). (B) Western blotting assays displayed the PTGES3 degradation in HCC cells treated with PTGES3-PROTAC at different time points (n = 3). (C) Western blotting analysis of the ubiquitinated levels of PTGES3 in HCC cells treated with PTGES3-PROTAC and/or MG132 (n = 3). (D) Western blotting analysis of the protein levels of PTGES3 in HCC cells treated with PTGES3-PROTAC and/or MG132 (n = 3). (E) CCK-8 assays were performed to test proliferation of HCC cells treated with FIP-2 or PTGES3-PROTAC (n = 3). (F) Western blotting assays showed the PTGES3 protein expression in HCC cells treated with FIP-2 or PTGES3-PROTAC (n = 3). (G) Detection of PTGES3 mRNA expression by qRT-PCR in HCC cells treated with FIP-2 or PTGES3-PROTAC (n = 3)