Fig. 13

Analysis of in-vitro assays performed on differentiated Saos-2 cells. The panels A–C depicts the visualization and quantification of the amount of calcium deposition using A taurine treatment doses, B homocysteine treatment doses, and C vitamin B12 treatment doses. In the plots non-treated Saos-2 cells grown in normal complete media are shown as negative control (NC) and the Saos-2 cells that underwent differentiation in osteogenic media are shown as positive control (PC). The results are expressed as mean ± SEM (n = 3)