Fig. 9

RUNX1 promotes LINC01094 expression and the regulative effect could be inhibited by Ro 5–3335. Notes A RUNX1 was predicted by the JASPAR database to bind with LINC01094 (top) and the potential binding region was visualized (bottom). B The expression of LINC01094 was positively correlated with that of RUNX1 according to the TCGA STAD database. C The association between the expression of LINC01094 and that of RUNX1 was validated by qRT-PCR. D A luciferase reporter assay was used to confirm that RUNX1 could promote the transcription of LINC01094. E The binding region between RUNX1 and LINC01094 was examined by a luciferase assay. F, G ChIP-qPCR indicated that RUNX1 could promote LINC0194 transcription by binding with site 2 and site 4 in the LINC01094 promoter. H, I RUNX1 could regulate CDKN1A expression by affecting LINC01094. J–R The effects of LINC01094 on malignant behaviors in vitro (J–O) and in vivo (P–S) could be affected by RUNX1 expression