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Fig. 2 | Biology Direct

Fig. 2

From: TFAP2C-mediated transcriptional activation of STEAP3 promotes lung squamous cell carcinoma progression by regulating the β-catenin pathway

Fig. 2

Knockdown of STEAP3 inhibits the proliferation and metastasis of LUSC cells. (A) The SK-MES-1 and NCI-H520 cells were transfected with the recombinant plasmids pLKO.1-sh-STEAP3 and pcDNA-3.1-STEAP3, respectively. Following the selection of stable transfectants, the protein levels of STEAP3 were quantified by western blot analysis. β-actin was employed as the internal control. (B) The assessment of cellular proliferation at various time points was performed using the CCK-8 assay following the knockdown and overexpression of STEAP3. (C) The colony formation assay was employed to evaluate the colony-forming ability of LUSC cells following STEAP3 knockdown and overexpression. (D) Cell cycle distribution was analyzed by flow cytometry. (E) The capacity of the cells to migrate was evaluated using the wound healing assay. Scale bar represents 200 μm. (F) The transwell assay was employed to evaluate the cell migration and invasion ability. Scale bar represents 100 μm. **P < 0.01

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Biology Direct

ISSN: 1745-6150

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