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Fig. 5 | Biology Direct

Fig. 5

From: TFAP2C-mediated transcriptional activation of STEAP3 promotes lung squamous cell carcinoma progression by regulating the β-catenin pathway

Fig. 5

STEAP3 overexpression partially abolishes TFAP2C knockdown-mediated anti-tumor effects. (A) The assessment of cellular proliferation at various time points was performed using the CCK-8 assay following co-transfection. (B) Cell cycle distribution was analyzed by flow cytometry. (C) The capacity of the cells to migrate was evaluated using the wound healing assay. Scale bar represents 200 μm. (D) The transwell assay was employed to evaluate the cell migration and invasion ability. Scale bar represents 100 μm. (E) The expression levels of E-cadherin and N-cadherin were analyzed by immunofluorescence analysis. Scale bar represents 50 μm. (F) The protein levels of β-catenin in the cytoplasm and nucleus were quantified by western blot analysis. β-actin was employed as the internal control. (G) The protein levels of c-myc and cyclin D1 were quantified by western blot analysis. β-actin was employed as the internal control. *P < 0.05 and **P < 0.01

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