Fig. 9

Clinical significance, prognostic value, and biological function of DCAF13 in HCC. (A) The mRNA levels of the DCAF13 were determined in HCC tissues and adjacent normal samples. (B) Kaplan–Meier survival curves according to DCAF13 expression in HCC specimens. (C) Western blot analysis of HIF-1α, VEGF, NOTCH-1, β-catenin, c-Myc, and CCND1 in Huh7 and HepG2 cells. (D) CCK-8 assay was used to evaluate the cell proliferation of Huh7 and HepG2 cells. (E) Colony formation assay was used to evaluate the cell proliferation of Huh7 and HepG2 cells. (F) Representative pictures of lumen formation assay