Fig. 7

Expression of NDUFS2 was decreased in AMs from COPD mice. a Eight-week-old C57BL/6 mice were exposed to CS to construct COPD mouse model, while the littermates exposed to room air for the same period served as control (n = 6). The protein levels of NDUFS2 (red) and F4/80 (green) in these mice lungs were measured by immunofluorescence staining (Scale bars = 50 μm). The overall expression levels of NDUFS2 and F4/80 in mouse lung sections were quantified as the red and green fluorescence intensity, respectively. The expression level of NDUFS2 in pulmonary macrophages (marked by F4/80) was quantified as the proportion of NDUFS2⁺F4/80⁺ cells in total F4/80.⁺ cells. **P < 0.01 vs. Ctrl (Student’s t-test). Scale bars = 50 μm. b, c Eight-week-old C57BL/6 mice were exposed to CS to construct COPD mouse model, while the littermates exposed to room air for the same period served as control (n = 6). The mRNA (b) and protein (c) levels of Ndufs2 were measured by qPCR and immunofluorescence staining in the primary AMs isolated from each mouse. The overall expression levels of NDUFS2 and HSP60 in AMs were quantified as the red and green fluorescence intensity, respectively. The representative images of AMs at low magnification were displayed (Scale bars = 20 μm) in the white solid box at bottom left corner of every enlarged image at higher magnification (Scale bars = 10 μm). The protein levels of NDUFS2 (red) in AMs (marked by HSP60, green) was quantified as red fluorescence intensity. **P < 0.01 vs. Ctrl (Student’s t-test)