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Fig. 1 | Biology Direct

Fig. 1

From: Bi-targeting of thioredoxin 1 and telomerase by thiotert promotes cell death of myelodysplastic syndromes and lymphoma

Fig. 1

Thiotert inhibited the expression of TERT and TRX1 in MDS and lymphoma cells. (A) Theoretical mechanism diagram of thiotert. (B, C) Relative mRNA expression levels of Tert or Trx1 upon different concentrations of thiotert induction in SKM-1 (B) and U-937 (C) cells, and the GAPDH gene as the reference for quantitative analysis. (D) Western blotting analysis of TERT, TRX1, and TXNIP expression treated with different periods and increased concentrations of thiotert. The quantification data are shown below, the protein levels were normalized to the β-actin. (E) Enzyme activity analysis of TRXR upon thiotert treatment in SKM-1 and U-937 cells. (F) Western blotting analysis showed the effect of GSH and MitoQ on the expression of TERT and TRX1 induced by thiotert for 12 h, and the quantification data were shown on the right. *P < 0.05, **P < 0.01, ***P < 0.001

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Biology Direct

ISSN: 1745-6150

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