Fig. 3

Thiotert treatment induced oxidative stress in MDS and lymphoma cells. (A, B) Flow cytometric analysis showing the cytoplasm ROS or mitochondrial ROS levels induced by thiotert measured by DCF-DA and MitoSOX staining. The histograms of quantification data are shown on the right. (C) Western blot of MnSOD expression treated with different concentrations or temporal gradient of thiotert, and quantification data are shown on the right. (D) Representations of cytoplasm ROS levels in SKM-1 and U-937 cells subjected to the thiotert treatment which was pretreated with GSH and MitoQ. Hoechst was used for nuclear staining, and quantification data were shown on the right. (E-G) Cell viability of SKM-1 cells treated with thiotert in the presence or absence of GSH (1mM), MitoQ (0.5µM), and NAC (2µM). *P < 0.05, **P < 0.01, ***P < 0.001