Fig. 4

Thiotert treatment induces DNA damage in MDS and lymphoma cells. (A) Representative images of γH2AX immunostaining upon various concentrations of thiotert treatment for 12 h in SKM-1 cells, and the histogram shows quantification data of DNA damage (γH2AX) signals. (B) Representative images of TRF2 immunostaining after thiotert (40 µM) administration for 12 h, quantification data were shown on the right. (C-E) Western blotting analysis of DNA damage-related protein in MDS and lymphoma cells induced by thiotert in different concentrations or temporal gradients, and the quantification data of γH2AX expression, all the protein signals were normalized to β-actin. (F) The result of DNA electrophoresis. 5-Fu was used as a positive control. (G) Western blotting analysis of γH2AX expression when exposed to a thiotert treatment (40 µM) with the presence of GSH (1 mM) and MitoQ (0.5 µM) in SKM-1 cells. *P < 0.05, **P < 0.01, ***P < 0.001