Fig. 2

Immune Cell Infiltration in Pancreatic Cancer Samples with High OSBPL3 Expression. (A) Representative immunofluorescence co-staining images of human pancreatic cancer samples in the high OSBPL3 expression group (High-OSBPL3) and the low OSBPL3 expression group (Low-OSBPL3), magnification: 20X. (B) Heatmap showing the expression of genes associated with M2 macrophage polarization and regulatory T cells (Tregs) in high and low OSBPL3 expression samples across the GSE62452, GSE16515, GSE32676, and GSE101462 datasets. Colors represent gene expression levels, and “+” indicates the high-expression group. (C) Correlation between OSBPL3 expression and immune cell infiltration predicted by algorithms such as ESTIMATE and EPIC across various pancreatic cancer datasets. Rows represent different datasets, and colors indicate the strength and direction of the correlation. (D) Subcutaneous tumor images, tumor weight and growth curve statistics, and representative immunofluorescence co-staining images of T-cell marker genes following OSBPL3 overexpression in B6/C57 mice. Magnification: 20X. (E) Expression distribution of OSBPL3 and the macrophage marker gene MRC1 in the pancreatic cancer spatial transcriptomics dataset GSE111672. The bar chart on the left shows the proportion of MRC1-positive cells in areas with high and low OSBPL3 expression. (F) Expression of OSBPL3 in the pancreatic cancer spatial transcriptomics dataset GSM6177618 and the tumor and immune cell expression distribution derived from immune cell deconvolution analysis based on “SpaCET”